Test: Can ELISA kits directly detect specimens?

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Kaixin micro test

Test probe P100-M3

Serum plasma is a very common specimen in ELISA scientific research. Shanghai Jinma technical experts said that not all species and indicators of the kit are suitable for direct detection of serum plasma. So how do you know if the elisa kit can directly detect specimens? Take a look at the following small tests: 1. If the manufacturer does not provide a buffer specifically for serum plasma samples, then just add the serum of the tested species to the sample with the known concentration of the test protein. By using a known concentration of the indicator protein to be added to the PBS buffer and detecting the comparison, it is known whether the kit can be directly used to measure serum plasma samples. 2. If the manufacturer does not provide a buffer for serum plasma samples, but there is a general or uniform dilution, then just add the sample with the known concentration of the test protein to the serum of the tested species. The known concentration of the indicator protein to be tested is added to the uniform buffer provided by the manufacturer for simultaneous detection, and it can also be found whether the kit can be directly used for detecting serum plasma samples. 3. If the manufacturer provides a buffer specially used for serum plasma samples, the ELISA test kit can be directly added to the serum of the tested species with a known concentration of the test target protein and separately loaded with the buffer according to the instructions. come to conclusion.

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